About Singh101
I have been a Research Associate for 5 and a half years specialising in molecular and cell biology. My work involved investigating role of genes implicated in atherosclerosis. These were mainly apolipoprotein E, ABCA1, LPL and scavenger receptors. Currently I am working part time in the health service.
My PhD. Thesis Title: Regulation of Apolipoprotein E gene expression by Transforming growth factor-beta in THP-1 cells.
My M.S.c. Research Project: Cloning ‘von willebrand factor C repeat’ of the N-propeptide of type I collagen.
Tissue culture: Experience in several mammalian cell lines (THP-1, U937, Hep3B, J774.2, RAW 264.7, primary monocytes and macrophages from buffy coats), determination of cell viability and concentration, cell cryopreservation, isolation of monocytes/macrophages from peripheral blood, isolation of peritoneal macrophages from mice. Extensive experience in cell maintenance, procedures, growth and morphology.
Skills
Molecular biology: DNA and RNA purification techniques including gel electrophoresis, transformations, transfection assays, restriction digests, ligations, Northern blots, RNA interference of gene expression (RNAi), reporter gene assays, electrophoretic mobility shift assay (EMSA) and micro arrays. Experience in reverse transcriptase and real time PCR, practical experience of gene cloning and transformation techniques. Some experience in flow cytometry.
Immunological methods: Antibody use in various western blots, kinase and activity assays and assays, immunoprecipitation with Protein A/G Beads, general detection methods.
Biochemical methods: Working with radioactivity namely 14C, gamma ATP and p32.
If you would like to get in touch email me at”info AT singh101.com”
